This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) purified IgG in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide.
Alternative names of antibody target:
Western blot analysis (5-10 µg/ml) and immunoprecipitation. The antibody detects a 12.0 kDa protein corresponding to the apparent molecular weight of cytochrome c.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Cytochrome c purified from human heart
How to handle this antibody:
The antibody solution should be gently mixed before use.
The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate caspases responsible for apoptosis.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.