Human CellExp™ Serpin E2 / PN1, human recombinant

• Catalog number
  7515-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, PI-7, Protease nexin 1, PN-1, Serpin E2, SerpinE2, PI7
• Alternative_names
  SERPINE2, Glia-derived nexin, GDN, Peptidase inhibitor 7, PI-7, Protease nexin 1, PN-1, Serpin E2, SerpinE2, PI7
• Description
  ≥92% pure, active, human cell expressed, human recombinant Serpin E2 / PN1 - the primary physiological inhibitor of uPA and tPA.
• Recombinant
  Yes
• Source
  HEK293 cells
• Purity by SDS PAGE
  ≥92%
• Assay
  SDS-PAGE
• Biological activity
  Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval – WRK (Dnp) - NH2.
• Molecular Weight
  This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 42.7 kDa. The predicted N-terminus is Ser 20. DTT-reduced Protein migrates as 45-48 kDa in SDS-PAGE due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in 20 mM NaAc, 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Background Information
  SERPINE2 is also known as Glia-derived nexin (GDN), Peptidase inhibitor 7 (PI7), Protease nexin 1(PN1). SERPINE2 is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). PAI-1 / GDN is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis. Defects in Serpin E1 / PN1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   Serpin   PN1  
• Gene symbol
  SERPINE2, SCN9A
• Short name
  CellExp™ Serpin E2 / PN1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ Serpin E2 / PN1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8951
• Gene
  SERPINE2
• Long gene name
  serpin family E member 2
• Synonyms gene
  • PI7
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Synonyms
  • PN1
  • GDN
  • PNI
  • nexin
• Synonyms name
  • glial-derived nexin 1
• GenBank acession
  • M17783
• Locus
  2q36.1
• Discovery year
  1994-09-01
• Entrez gene record
  5270
• Pubmed identfication
  • 7665170
  • 24172014
• RefSeq identity
  • NM_006216
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000133163
• Locus Specific Databases
  • LRG_645

Gene info

• Identity
  HGNC:10597
• Gene
  SCN9A
• Long gene name
  sodium voltage-gated channel alpha subunit 9
• Synonyms gene name
  • sodium channel, voltage-gated, type IX, alpha polypeptide
  • sodium channel, voltage-gated, type IX, alpha subunit
  • sodium channel, voltage gated, type IX alpha subunit
• Synonyms
  • Nav1.7
  • PN1
  • NE-NA
  • NENA
  • ETHA
• GenBank acession
  • X82835
• Locus
  2q24.3
• Discovery year
  1996-04-12
• Entrez gene record
  6335
• Pubmed identfication
  • 7720699
  • 10198179
  • 16382098
• RefSeq identity
  • NM_002977
• Classification
  • Sodium voltage-gated channel alpha subunits
• VEGA ID
  OTTHUMG00000154044
• Locus Specific Databases
  • LRG_369

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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