-
Other name
Mitogen-activated protein kinase 9,MAP kinase 9,MAPK 9,Stress-activated protein kinase 1a,SAPK1a,Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,MAPK9,JNK2, PRKM9,SAPK1A
-
Immunogen
Recombinant human Mitogen-activated protein kinase 9 protein (1-424AA)
-
Verified reactivity
Human, Mouse
-
Verified applications
ELISA,WB,IP. Recommended dilution:WB:1:1000-1:5000,IP:1:200-1:2000
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Protein number
P45984
-
Gene number
Please refer to GenBank
-
NCBI number
Please refer to NCBI
-
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
-
Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
-
Use before
1 year
-
Shipping requirements
Blue ice
-
Estimated production time
3-7 business days
-
Supplementary information
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
-
Notes
For research use only. Not for diagnostic procedures.
-
-
Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
MAPK9
-
Short name
MAPK9 Polyclonal Antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Label
Unconjugated
-
Alternative name
mitogen-activated protein kinase 9 polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
mitogen-activated protein kinase 9, JNK-55 and JNK2 and JNK2A and JNK2ALPHA and JNK2B and JNK2BETA and p54a and p54aSAPK and PRKM9 and SAPK and SAPK1a, MAPK9 and IDBG-62183 and ENSG00000050748 and 5601, transferase activity, nuclei, Mapk9 and IDBG-167617 and ENSMUSG00000020366 and 26420, MAPK9 and IDBG-640895 and ENSBTAG00000004709 and 534125
-
