Human Recombinant TNF-beta/LTA
Lymphotoxin alpha, LT, TNFB, TNFSF1.
Mediates a large variety of inflammatory, immunostimulatory, and antiviral responses
Purity by SDS-PAGE
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is
20.9 kDa (192 aa, 35-205 aa + His-Tag)
Physical form description
0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol.
TNF-beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is highly inducible, secreted, and exists as homotrimeric molecule. It forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. It is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
H. sapiens recombinant tumor necrosis factor-b/lymphotoxin alpha (tumor necrosis factor superfamily, member 1)
Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943, lymphotoxin alpha (TNF superfamily, member 1), LT and TNFB and TNFSF1, LTA and IDBG-302490 and ENSG00000226979 and 4049, protein binding, Extracellular, Lta and IDBG-177399 and ENSMUSG00000024402 and 16992, LTA and IDBG-634171 and ENSBTAG00000000016 and 280845