B-TeZ IAC Biotin Vitamin affinity column
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Catalog number
BTSA317005
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Price
Please ask
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Size
10 columns
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ELISA category
Vitamin affinity column
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ELISA detection limits
0.01 - 5μg
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ELISA sensitivity
0.01 μg
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ELISA s description
10 Immuno affinity columns with specific antibodies binding Biotin (Vitamin B7, Vitamin H). For enrichment or purification.
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Research area interests
Vitamin Analysis
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Package form
Column size 3mL
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Antigen
Biotin
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Reacts with
Biotin
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Tested applications
B-TeZ IAC Biotin is an immuno affinity column (IAC) for enrichment and isolation of Biotin. The IAC can be used after extraction of the analytes.
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Specificity
Biotin
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Crossreactivity
N/A
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Preservative materials
If applicable mentioned in the product data sheet
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Storage recommendations
12 month
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Shipping and storage conditions
> 0 to +37°C
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Long term storage
2-8°C
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Assay principle
Enrichment of analytes via immuno affinity chromatography
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Included in the standard package
N/A
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Not included in the standard package
N/A
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Samples to be analyzed
food and feed crop probes
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PubMed citations
See the datasheet
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Technical datasheet
Contact us to receive the datasheet
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Representative figures
No Available
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Warnings
For Research Use only.
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Test
A high affinity purification column was use to purify B-TeZ IAC Vitamin column by biotez by chromatographic size exclusion.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
MeSH Data
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Name
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Concept
Scope note:
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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