-
Clone
9G10
-
Immunogen
Purified Grp94 isolated from chicken oviducts
-
Antibody s full description
Rat Anti-Chicken GRP94 Monoclonal IgG2a Antibody, Clone: 9G10: Biotin
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
HSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody
-
Raised in
Rat
-
Antibody s target
GRP94
-
Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking
-
Brandname
None
-
Antibodies applications
WB, ICC/IF, IP, FCM
-
Antibody s reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig (Cavia porcellus), Hamster, Horse, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
-
Antibody s dilutions
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
Purity
Protein G Purified
-
Antibody buffer for storage
PBS pH7.2, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
-
Antibody in cell
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen , Melanosome
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
-
Bibliography
1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128. 2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98. 3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262:8875-8883. 4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9):5643-5649. 5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338. 6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269. 7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637. 8. Allen S. et al. (2000) Blood 96(2): 560-568. 9. Sato K et al. (2001) Blood 98(6): 1852-1857. 10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5):371-378. 11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858. 12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386. 13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104. 14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
-
Released date
1-Jun-2007
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
NCBI number
NP_003290.1
-
Gene number
7184
-
Protein number
P14625
-
Antibody s datasheet
Contact our support service
-
Representative figure link
-
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Western Blot analysis of Human HeLa cell lysates showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat. Rat Anti-GRP94 Antibody [9G10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-105) Rat Anti-GRP94 Antibody [9G10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-105) Rat Anti-GRP94 Antibody [9G10] used in Western Blot (WB) on Human HeLa cell lysates (SMC-105)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.2
-
Stock availability
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin name
Rattus norvegicus
