NFNB, bacterial recombinant
-
Catalog number7830-100
-
PricePlease ask
-
Size100 ug
-
-
SynonymsDihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.
-
Alternative_namesDihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.
-
DescriptionAn enzyme for activating prodrugs in antibody directed enzyme prodrug therapy
-
RecombinantYes
-
SourceE. coli.
-
Purity by SDS PAGE≥95%
-
AssaySDS-PAGE
-
Biological activitySpecific activity: > 2 units/ml One unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6.7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC
-
ResultsSpecific activity: > 2 units/ml
-
Unit DefinitionOne unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6.7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC
-
Molecular Weight26.0 kDa (237 aa, 1-217 aa + His Tag)
-
Storage Temp-20°C
-
Shippinggel pack
-
Shelf Life12 months
-
Concentration1 mg/ml
-
AppearanceLiquid
-
Physical form description1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 50 mM NaCl
-
Background InformationNFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
-
Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Short nameNFNB, bacterial recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative nameNFNB, bacterial Rec.
-
Alternative techniquerec
-
MeSH Data
-
Name
-
ConceptScope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
-
Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
-
Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data