Krebs-Ringer Bicarbonate Buffer w/ Calcium chloride, 10mM HEPES buffer and 0.2% Bovine Serum Albumin w/o Glucose and Sodium bicarbonate

• Catalog number
  TS1143-8X5L
• Price
  Please ask
• Size
  8X5L

• Description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Group
  Sera
• About
  Serum should be stored at +5°C

• Gene target
  Krebs-Ringer   Bicarbonate   Calcium   chloride   10mM   Albumin   w/o   Glucose   Sodium   bicarbonate  
• Short name
  Krebs-Ringer Bicarbonate w/ Calcium chloride, 10mM 2% Serum Albumin w/o Glucose Sodium bicarbonate
• Technique
  serum
• Species
  Bovine, Bos Taurus genes are available form the Bovine Genome database.
• Alternative name
  Krebs-Ringer Bicarbonate solution w/ Calcium chloride, 10mM HEPES solution and 0.2% Bovine blood serum Albumin w/o Glucose and Sodium bicarbonate
• Alternative technique
  sera
• Tissue
  serum

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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