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Long name
Rabbit Antibody against Human BUB1B, HRP
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Synonym name
BUB1B Human Antibody
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Species reactivity
Human
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Tested applications recommended dilutions
ELISA
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Conjugation
HRP
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Antibody isotype
IgG
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UniProt ID
O60566
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein (230-394AA)
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Immunogen species
Human
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Alias
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta,MAD3/BUB1-related protein kinase,hBUBR1,Mitotic checkpoint kinase MAD3L,Protein SSK1,BUB1B,BUBR1,MAD3L,SSK1
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Background
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
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Clonality
Polyclonal antibody
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Shipping and storage
BUB1B Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugation
HRP Conjugated
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
BUB1B-PAK6, BUB1B
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Short name
Anti-BUB1B
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Technique
anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
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Host
Rabbit, Rabbits
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Label
HRP
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Species
Human, Humans
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Alternative name
BUB1B Antibody,HRP conjugated
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Alternative technique
antibodies, rabbit-anti
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Alternative to gene target
budding uninhibited by benzimidazoles 1 homolog beta (yeast), Bub1A and BUB1beta and BUBR1 and hBUBR1 and MAD3L and MVA1 and SSK1, BUB1B and IDBG-6103 and ENSG00000156970 and 701, transferase activity, nuclei, Bub1b and IDBG-198179 and ENSMUSG00000040084 and 12236, BT.25661 and IDBG-646208 and ENSBTAG00000007237 and 100851213,537027
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