Recombinant Human RANK L/TRANCE/TNFSF11 Protein(N-6His)

• Catalog number
  PKSH032986-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  CD254; ODF; OPGL; RANKL; TNFSF11; CD254; Osteoclast differentiation factor; Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11
• Activity
  Measured by its ability to binding SF11A used funtional ELISAThe ED50 for this effect is less than 10 ug/ml.
• Sequence
  Ile140-Asp317
• Fusion tag
  N-6His
• Accession
  O14788
• Expressed Host
  E.coli
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞90% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks
• Mol Mass
  22.4 kDa
• AP Mol Mass
  20 kDa
• Formulation
  Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
• Background
  CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  RANK   L/TRANCE/TNFSF11   Protein   N-6His  
• Gene symbol
  TNFSF11, TNFRSF11A, ANKFY1
• Short name
  Recombinant RANK L/TRANCE/TNFSF11 Protein(N-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens RANK L/TRANCE/tumor necrosis factor (ligand) superfamily, member 11 Protein(N-6His)
• Alternative technique
  rec
• Alternative to gene target
  tumor necrosis factor (ligand) superfamily, member 11, CD254 and hRANKL2 and ODF and OPGL and OPTB2 and RANKL and sOdf and TRANCE, TNFSF11 and IDBG-28393 and ENSG00000120659 and 8600, tumor necrosis factor receptor superfamily binding, Extracellular, Tnfsf11 and IDBG-182101 and ENSMUSG00000022015 and 21943, TNFSF11 and IDBG-628731 and ENSBTAG00000008924 and 513836

Gene info

• Identity
  HGNC:11926
• Gene
  TNFSF11
• Long gene name
  TNF superfamily member 11
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 11
• Synonyms
  • TRANCE
  • RANKL
  • OPGL
  • ODF
  • CD254
• GenBank acession
  • AF013171
• Locus
  13q14
• Discovery year
  1998-12-04
• Pubmed identfication
  • 9312132
  • 9367155
• Classification
  • Tumor necrosis factor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000016807

Gene info

• Identity
  HGNC:11908
• Gene
  TNFRSF11A
• Long gene name
  TNF receptor superfamily member 11a
• Synonyms gene
  • PDB2
  • LOH18CR1
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 11a, activator of NFKB
  • Paget disease of bone 2
  • loss of heterozygosity, 18, chromosomal region 1
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• Synonyms
  • RANK
  • CD265
  • FEO
  • ODFR
  • TRANCE-R
• Synonyms name
  • osteoclast differentiation factor receptor
  • receptor activator of nuclear factor kappa B
  • familial expansile osteolysis
  • TRANCE receptor
• GenBank acession
  • AF018253
• Locus
  18q21.33
• Discovery year
  1998-12-04
• Entrez gene record
  8792
• Pubmed identfication
  • 9367155
  • 10615125
• Classification
  • CD molecules
  • Tumor necrosis factor receptor superfamily
• VEGA ID
  OTTHUMG00000132779
• Locus Specific Databases
  • LRG_194

Gene info

• Identity
  HGNC:20763
• Gene
  ANKFY1
• Long gene name
  ankyrin repeat and FYVE domain containing 1
• Synonyms
  • ANKHZN
  • KIAA1255
  • ZFYVE14
  • BTBD23
  • RANK-5
• Synonyms name
  • rabankyrin-5
• GenBank acession
  • AB033081
• Locus
  17p13.2
• Discovery year
  2003-04-29
• Entrez gene record
  51479
• Pubmed identfication
  • 10940552
  • 17273843
• RefSeq identity
  • NM_016376
• Classification
  • Zinc fingers FYVE-type
  • BTB domain containing
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000177727

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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