Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate

  • Catalog number
    AT083-5X10X1L
  • Price
    Please ask
  • Size
    5X10X1L
  • Description
    Nutrient Mixture F-10 Ham, With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. It was designed for clonal growth of CHO cells and chick embryo cells under serum free conditions. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Off-white to Creamish white, homogenous powder.
  • PH without Sodium Bicarbonate
    5.60 -6.20
  • PH with Sodium Bicarbonate
    6.40 -7.00
  • Osmolality without Sodium Bicarbonate
    320.00 -360.00
  • Osmolality with Sodium Bicarbonate
    340.00 -380.00
  • Cultural Response
    The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content
    NMT 5EU/ml
  • Solubility
    Clear solution at 15.8 gms/L.
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Ordering
    To order Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, please use the Cat. Nr.AT083-5X10X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Nutrient Mixture F-10 Ham With L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
  • Gene symbol
    F10, F10-AS1, HLA-F, H3C11, IGHV1-69-2, SNAR-F, PIRC49, KLRC4, SNORD115-10
  • Short name
    Nutrient Mixture F-10 Ham With L-Glutamine 25mM Without Sodium bicarbonate
  • Alternative name
    Nutrient Mixture F-10 Ham including L-Glutamine and 25mM HEPES solution Without Sodium bicarbonate
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    F10 antisense RNA 1
  • Synonyms gene name
    • F10 antisense RNA 1 (non-protein coding)
  • Locus
  • Discovery year
    2011-05-09
  • Classification
    • Antisense RNAs
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
  • Discovery year
    2008-06-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 49
  • Locus
    10
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-09-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
Gene info
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