Hepa 1-6 Cell Line

• Catalog number
  EP-CL-0105
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  Hepa 1-6
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  NA
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  Tested and found negative for ectromelia virus(mousepox)
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1mM Sodium Pyruvate (PB180422)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  NA
• Effects
  NA
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Albumin, Alpha Fetoprotein(Afp, Alpha-Fetoprotein); Albumin; Alpha 1 Antitrypsin(Alpha-1-Antitrypsin); Amylase
• Applications
  Transfection host.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Hepa   1-6   Line  
• Gene symbol
  TRL-CAA6-1, TRQ-TTG10-1, TRY-GTA6-1, TRI-AAT6-1, TRL-AAG6-1, TRS-GCT6-1, TRC-GCA6-1, TRG-TCC6-1, TRR-TCG6-1
• Short name
  Hepa 1-6 Line
• Species
  Mus musculus, Mouse
• Alternative name
  Hepa 1-6 cellular Line
• Tissue
  Liver

Gene info

• Identity
  HGNC:34560
• Gene
  TRL-CAA6-1
• Long gene name
  tRNA-Leu (anticodon CAA) 6-1
• Synonyms gene
  • TRNAL3
• Synonyms gene name
  • transfer RNA leucine 3 (anticodon CAA)
  • transfer RNA-Leu (CAA) 6-1
• Synonyms
  • tRNA-Leu-CAA-6-1
• GenBank acession
  • HG983887
• Locus
  1q23.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189003
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34569
• Gene
  TRQ-TTG10-1
• Long gene name
  tRNA-Gln (anticodon TTG) 10-1
• Synonyms gene
  • TRNAQ5
• Synonyms gene name
  • transfer RNA glutamine 5 (anticodon UUG)
  • transfer RNA-Gln (TTG) 10-1
• Synonyms
  • tRNA-Gln-TTG-10-1
• GenBank acession
  • HG983752
• Locus
  6
• Discovery year
  2008-08-29
• Entrez gene record
  100189010
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Gene info

• Identity
  HGNC:34782
• Gene
  TRY-GTA6-1
• Long gene name
  tRNA-Tyr (anticodon GTA) 6-1
• Synonyms gene
  • TRNAY8
• Synonyms gene name
  • transfer RNA tyrosine 8 (anticodon GUA)
  • transfer RNA-Tyr (GTA) 6-1
• Synonyms
  • tRNA-Tyr-GTA-6-1
• GenBank acession
  • HG984083
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189219
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34940
• Gene
  TRI-AAT6-1
• Long gene name
  tRNA-Ile (anticodon AAT) 6-1
• Synonyms gene
  • TRNAI22
• Synonyms gene name
  • transfer RNA isoleucine 22 (anticodon AAU)
  • transfer RNA-Ile (AAT) 6-1
• Synonyms
  • tRNA-Ile-AAT-6-1
• GenBank acession
  • HG983852
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189372
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34998
• Gene
  TRL-AAG6-1
• Long gene name
  tRNA-Leu (anticodon AAG) 6-1
• Synonyms gene
  • TRNAL39
• Synonyms gene name
  • transfer RNA leucine 39 (anticodon AAG)
  • transfer RNA-Leu (AAG) 6-1
• Synonyms
  • tRNA-Leu-AAG-6-1
• GenBank acession
  • HG983878
• Locus
  20
• Discovery year
  2008-08-29
• Entrez gene record
  100189429
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Gene info

• Identity
  HGNC:34781
• Gene
  TRS-GCT6-1
• Long gene name
  tRNA-Ser (anticodon GCT) 6-1
• Synonyms gene
  • TRNAS16
• Synonyms gene name
  • transfer RNA serine 16 (anticodon GCU)
  • transfer RNA-Ser (GCT) 6-1
• Synonyms
  • tRNA-Ser-GCT-6-1
• GenBank acession
  • HG984031
• Locus
  6p22.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189218
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34741
• Gene
  TRC-GCA6-1
• Long gene name
  tRNA-Cys (anticodon GCA) 6-1
• Synonyms gene
  • TRNAC14
• Synonyms gene name
  • transfer RNA cysteine 14 (anticodon GCA)
  • transfer RNA-Cys (GCA) 6-1
• Synonyms
  • tRNA-Cys-GCA-6-1
• GenBank acession
  • HG983693
• Locus
  3q22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189179
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35086
• Gene
  TRG-TCC6-1
• Long gene name
  tRNA-Gly (anticodon TCC) 6-1
• Synonyms gene
  • TRNAG35P
• Synonyms gene name
  • transfer RNA glycine 35 (anticodon UCC) pseudogene
  • transfer RNA-Gly (TCC) 6-1
• Synonyms
  • tRNA-Gly-TCC-6-1
• GenBank acession
  • HG983831
• Locus
  18q21.31
• Discovery year
  2008-08-29
• Entrez gene record
  100189511
• RefSeq identity
  • NG_008569
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Gene info

• Identity
  HGNC:34584
• Gene
  TRR-TCG6-1
• Long gene name
  tRNA-Arg (anticodon TCG) 6-1
• Synonyms gene
  • TRNAR6
• Synonyms gene name
  • transfer RNA arginine 6 (anticodon UCG)
  • transfer RNA-Arg (TCG) 6-1
• Synonyms
  • tRNA-Arg-TCG-6-1
• GenBank acession
  • HG983620
• Locus
  9q31.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189025
• Classification
  • Cytoplasmic transfer RNAs

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