96-Well Ras Activation ELISA test kit (Chemiluminescent)

  • Catalog number
    STA-441
  • Price
    Please ask
  • Size
    96 assays
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    Upon receipt, aliquot and store Raf-1 RBD at -80ºC and avoid freeze/thaw. Aliquot and store the Anti-pan-Ras Antibody, Secondary Antibody, GTPγS, and GDP components at -20ºC and avoid freeze/thaw. Store all other components at 4ºC until their expiration dates.
  • SDS or safty data sheet
    more (STA-441) GHS.pdf
  • Type of reagent
    Cell Signaling and Protein Biology
  • Related techniques
    Cell Signaling and Protein Biology, G Protein Signaling / Small GTPase Assays and Reagents, Small GTPase Research Tools, Small GTPase Activation ELISA test kits, Ras Activation ELISA, Chemiluminescent
  • Use of the reagent
    Our 96-Well Ras Activation ELISA test kit uses the Raf1 RBD (Rho binding domain) bound to a 96-well plate to selectively pull down the active form of Ras from purified or endogenous samples. The captured GTP-Ras is then detected by a pan-Ras antibody and HRP-conjugated secondary antibody.
  • Cellular use
    Raf1 RBD detects only the active form of Ras
  • Protocol information
    Convenient 96-well format for use with any plate-based luminometer
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,12
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Storage
    Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
  • Group
    Enzyme-Linked Immunosorbent Assay kits
  • Gene target
  • Short name
    96- Ras Activation ELISA test kit (Chemiluminescent)
  • Technique
    activation, ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    96-Well Ras Activation Enzyme-linked immunosorbent assay test reagent (Chemiluminescent)
  • Alternative technique
    elisas, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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