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Long name
Mouse Monoclonal Antibody against Human Lamin C, Clone: EM-11
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Antigen
Lamin C
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Other not determined
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
EM-11
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Applications
IP, WB, ICC
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Format conjugation
purified; unconjugated
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Specificity
The mouse monoclonal antibody EM-11 recognizes lamin C, intermediate filament protein of nuclear lamina._x000D_
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Research area
Transcription Regulation & Nuclear Proteins (Rodent), Transcription Regulation & Nuclear Proteins (Human), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human Lamin C, Clone: EM-11 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Lamin C is intermediate filament protein localized to the inner nuclear membrane. It is expressed predominantly in terminally differentiated cells and defines the shape and stability of nuclei in mammalian cells. Besides their structural roles, lamin proteins also regulate fundamental aspects of nuclear function and they cross-talk with cell signaling cascades and cell metabolism. Mutations of LMNA gene, encoding lamin A and C proteins, are often associated with pathogenesis of respective cell types, such as of heart myocytes._x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Shimi T, Kittisopikul M, Tran J, Goldman AE, Adam SA, Zheng Y, Jaqaman K, Goldman RD: Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy. Mol Biol Cell. 2015 Nov 5;26(22):4075-86.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
