Identification of CD19 (FITC) Human B cells associated approximately 10% of peripheral blood lymphocytes, 95,000 M.W. surface antigen. Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
Antibody come from
CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture.
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
Antigen-antibody binding interaction
Mouse anti CD19 antibody conjugated to FITC-CD2 antibody conjugated to PE Antibody
Antibody's suited for
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼ C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼ C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
1.An Improved Rosetting Assay for Detection of Human T Lymphocytes. Kaplan M.E., Clark C., J. Immunol. Methods 1974, 5,131 _x000B__x000B_2.Structural and functional characterization of the CD2 immunoadhesion domain. Evidence for inclusion of CD2 in an alpha-beta protein folding class. Recny M.A., Neidhardt E.A., Sayre P.H., Ciardelli T.L., Reinherz E.L., J. Biol. Chem. 1990 May 2;265(15):85419 _x000B__x000B_3. Partial deletions of the cytoplasm domain of CD2 result in a partial defect in signal transduction. Bierer B.E., Bogart R.E., Burakoff S.J., J. Immunol. 1990 Feb. :144(3):785 _x000B__x000B_4. Functional CD2 mutants unable to bind to, or be stimulated by, LFA-3. Wolff H.L., Burakoff S.J., Bierer B.E., J. Immunol. 1990 Feb. 1;144(4):1215-20 _x000B__x000B_5. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 _x000B__x000B_6. Functional Properties of CD19+ B Lymphocytes Positively Selected from Buffy Coats by Immunomagnetic Separation. Funderud S., Erikstien B., Asheim H.C., Nustad K., Stokke T., Blomhoff H.K., Holte H, Smeland E.B., Eur. J. Immunol. 1990 Ja;20(1):201-6 _x000B__x000B_7. Thymic B Cells from Myasthenia Gravis Patients are Activated B Cells. Phenotypic and Functional Analysis. Leprince C., CohenKaminsky S., Berrih-Aknin S., Vernet-Der Garabedian B., Treton D., Galanaud P., Richard Y., J. Immunol. 1990 Oct., 145(7):2115-22 _x000B__x000B_8. Prognostic Significance of CD34 Expression in Childhood B Precursor Acute Lymphocytic Leukemia: A Pediatric Onocology Group Study. Borowitz MJ, Shuster JJ, Civin CI, Carrol AJ, Look AT, Behm FG, Land VJ, Pullen DJ, Crist WM, J. Clin. Onol. 1990 Au;8(8):1389-98 _x000B__x000B_9. Biphenotypic Acute Leukemia in Adults. Sulak LE, Clare CN, Morale BA, Hansen KL, Montiel MM, Am. J. Clin. Path. 1990 Ju;94(1):54-8 _x000B__x000B_10. Intersection of the Complement and Immune Systems: A Signal Transduction Complex of the B Lymphocyte Containing Complement Receptor type 2 and CD19. Matsumoto AK, Kopicky-Burd J., Carter Rh, Tuveson DA, Tedder TF, Fearon, DT, J. Exp. Med. 1991 Jan. 173(1):55-64
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mouse anti CD19 antibody conjugated FITC-CD2 antibody conjugated PE
Antibody, Mouse, anti, FITC
Mouse antibody to CD19 molecule (Antibody to) coupled to fluorecein-CD2 molecule (Antibody to) coupled to peroxidase
Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and, CD2 molecule, LFA-2 and SRBC and T11, CD2 and IDBG-101417 and ENSG00000116824 and 914, protein homodimerization activity, Extracellular, Cd2 and IDBG-179684 and ENSMUSG00000027863 and 12481, CD2 and IDBG-634458 and ENSBTAG00000017256 and 497022