PDGF-AA Recombinant Protein
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Catalog number
40-157
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
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Other name of the recombinant
PDGF1, PDGF-A, PDGF1, Platelet-derived growth factor subunit A, PDGF-1, PDGF subunit A
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Recombinant in
E. coli
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SEQUENCE
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
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Activity
The ED50 was Biological activity was determined by the dose - dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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Keep at
The lyophilized PDGF-AA recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted PDGF-AA is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing PDGF-AA recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
77695917
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Accession nr
NP_002598.4
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Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
5154
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Gene name
platelet-derived growth factor alpha polypeptide
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Source
Homo sapiens
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Uniprot
P04085
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
PDGFA
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Short name
PDGF-AA Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
PDGF-AA Rec. Protein
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Alternative technique
rec
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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