Caspase-3 Antibody

• Catalog number
  R30359
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Caspase-3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P42574
• Purity
  Antigen affinity
• Description
  Caspase-3 is a protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.
• Immunogen
  An amino acid sequence from the C-terminus of mouse Caspase-3 (CSMLKLYAHKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.
• Storage
  After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• French translation
  anticorps

• Gene target
  Caspase-3  
• Gene symbol
  CASP1P3, CASP3, CASP3P1
• Short name
  Anti-Caspase-3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Caspase-3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:43777
• Gene
  CASP1P3
• Long gene name
  caspase 1 pseudogene 3
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine peptidase pseudogene 3
• Locus
  11q22.3
• Discovery year
  2012-02-24
• Entrez gene record
  100422634
• RefSeq identity
  • NG_024409

Gene info

• Identity
  HGNC:1504
• Gene
  CASP3
• Long gene name
  caspase 3
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine protease
  • caspase 3, apoptosis-related cysteine peptidase
• Synonyms
  • CPP32
  • CPP32B
  • Yama
  • apopain
• GenBank acession
  • BC016926
• Locus
  4q35.1
• Discovery year
  1996-07-22
• Entrez gene record
  836
• Pubmed identfication
  • 8780721
• RefSeq identity
  • NM_004346
• Classification
  • Caspases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000133681

Gene info

• Identity
  HGNC:43596
• Gene
  CASP3P1
• Long gene name
  caspase 3 pseudogene 1
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine peptidase pseudogene 1
• Locus
  1p31.1
• Discovery year
  2011-11-22
• Entrez gene record
  100131616
• RefSeq identity
  • NG_022722
• VEGA ID
  OTTHUMG00000009345

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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