Caspase-3 Antibody

• Catalog number
  R30358
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Caspase-3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P42574
• Purity
  Antigen affinity
• Description
  Caspase-3, also known as YAMA, CPP32 or APOPAIN, is a member of the cysteine-aspartic acid protease family known to interact with family members -8 and -9. It is encoded by the CASP3 gene. Tiso et al.(1996) used radiation hybrid mapping to localize the gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CASP3 in insect cells induced apoptosis. Coexpression of the two CASP3 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human CASP1 cleaved the CASP3 proenzyme into a proteolytically active form and that activated Caspase-3 cleaved PARP into the 85-kD apoptotic form.
• Immunogen
  An amino acid sequence from the C-terminus of the human protein (CAMLKQYADKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.
• Storage
  After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• French translation
  anticorps

• Gene target
  Caspase-3  
• Gene symbol
  CASP1P3, CASP3, CASP3P1
• Short name
  Anti-Caspase-3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Caspase-3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:43777
• Gene
  CASP1P3
• Long gene name
  caspase 1 pseudogene 3
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine peptidase pseudogene 3
• Locus
  11q22.3
• Discovery year
  2012-02-24
• Entrez gene record
  100422634
• RefSeq identity
  • NG_024409

Gene info

• Identity
  HGNC:1504
• Gene
  CASP3
• Long gene name
  caspase 3
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine protease
  • caspase 3, apoptosis-related cysteine peptidase
• Synonyms
  • CPP32
  • CPP32B
  • Yama
  • apopain
• GenBank acession
  • BC016926
• Locus
  4q35.1
• Discovery year
  1996-07-22
• Entrez gene record
  836
• Pubmed identfication
  • 8780721
• RefSeq identity
  • NM_004346
• Classification
  • Caspases
  • Small nucleolar RNA protein coding host genes
• VEGA ID
  OTTHUMG00000133681

Gene info

• Identity
  HGNC:43596
• Gene
  CASP3P1
• Long gene name
  caspase 3 pseudogene 1
• Synonyms gene name
  • caspase 3, apoptosis-related cysteine peptidase pseudogene 1
• Locus
  1p31.1
• Discovery year
  2011-11-22
• Entrez gene record
  100131616
• RefSeq identity
  • NG_022722
• VEGA ID
  OTTHUMG00000009345

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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