Caspase-3 Antibody (phospho-S12)
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Catalog number
F48684-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS pH 7.4 with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Caspase-3 (phospho-S12)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
DB, ELISA
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Recommended dilutions
Dot blot: 1:500
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Notes
Titration of the phospho-Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-Caspase-3 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P70677
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Purity
Antigen affinity
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Description
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
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Immunogen
This phospho-Caspase-3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS12 of mouse CASP3.
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Storage
Aliquot the phospho-Caspase-3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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French translation
anticorps
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Gene target
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Gene symbol
RPS12, MRPS34, PSMD7, HTR1B
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Short name
Anti-Caspase-3 (phospho-S12)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Caspase-3 (phospho-S12)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
ribosomal protein S12
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Synonyms
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GenBank acession
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Locus
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Discovery year
1991-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA protein coding host genes
- S ribosomal proteins
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VEGA ID
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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