PPI, papain, Affinity purified

• Catalog number
  AS09 547
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized in phosphate buffer saline
• Immunogen
  native papain isolated and purified from Carica papaya
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified IgG
• How to reconstitute
  For reconstitution please add 0.5 ml of sterile destilled water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
• Connected products
  AS09 545 | anti-PPI | papainAS09 546 | anti-PPI | papain (biotinylated)Plant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 - 1: 100 000 for techniques listed above
• Molecular weight expected аpparent
  38.9 kDa
• Verified reactivity
  Carica papaya
• Possible reactivity
  Carica papaya
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
• References
  to be added when available
• Scientific context
  Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
• Notes
  antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Test
  A high affinity purification column was use to purify PPI, papain, by agisera by chromatographic size exclusion.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.

• Gene target
  PPI   papain  
• Short name
  PPI, papain,
• Technique
  affinity, purified, purifications
• Alternative name
  PPI, papain, protein enriched
• Alternative technique
  pure

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