BCAT1 Antibody

• Catalog number
  R30615
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  BCAT1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the BCAT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This BCAT1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P54687
• Purity
  Antigen affinity
• Description
  Branched-chain Aminotransferase 1 is also know as BCT1. The gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. Lack of the enzyme BCAT1 can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.
• Immunogen
  An amino acid sequence from the C-terminus of human BCAT1 (KLASRILSKLTDIQYGR) was used as the immunogen for this BCAT1 antibody.
• Storage
  After reconstitution, the BCAT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  BCAT1  
• Gene symbol
  BCAT1
• Short name
  Anti-BCAT1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to BCAT1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:976
• Gene
  BCAT1
• Long gene name
  branched chain amino acid transaminase 1
• Synonyms gene
  • BCT1
• Synonyms gene name
  • branched chain aminotransferase 1, cytosolic
• Synonyms
  • BCATc
• Locus
  12p12.1
• Discovery year
  2001-06-22
• Entrez gene record
  586
• Pubmed identfication
  • 9165094
• RefSeq identity
  • NM_005504
• VEGA ID
  OTTHUMG00000169053

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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