Escherichia coli O127:H6 DNA ligase B (ligB) -E. coli
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Catalog number
GEN1234231.E.coli
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Price
Please ask
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Size
1000ug
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Long name
Recombinant Escherichia coli O127:H6 DNA ligase B (ligB)
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Alternative names
NAD-dependent DNA ligase LigB; DNA ligase B; NAD-dependent DNA ligase LigB; Polydeoxyribonucleotide synthase [NAD(+)] B
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Gene name
ligB
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Other gene names
ligB; ligB;
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General description
DNA ligase B (ligB) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
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Product category
Recombinant Proteins
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Expression system
E. coli
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Available also expressed in
E Coli ; Yeast ; Baculovirus ; Mammalian Cell
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Purity
Greater than 90% (determined by SDS-PAGE)
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Form
Lyophilized protein
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Storage
This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
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Applications
This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
DNA ligase B (ligB)
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Technique
dna
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Host
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. MBS Recombinant supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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Species
E. coli, E. coli
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Alternative name
Escherichia coli O127:H6 Desoxyribonucleic acid ligase B (ligB) -E. coli
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Alternative technique
escherichia
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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