Human Poly ADP Ribose Polymerase (PARP) CLIA Kit, 96 well plate

• Catalog number
  EKU08862
• Price
  Please ask
• Size
  10x96T

• Verified reactivity
  Homo sapiens (Human)
• Detection limits
  3.12-200 ng/mL
• Sensitivity limit
  0,11 ng/mL
• Samples to be analyzed
  Tissue homogenates, cell lysates and other biological fluids
• Assay duration
  2 hours, 40min
• Verified applications
  CLIA
• Assay class
  Sandwich
• ELISA detection
  Chemiluminescent
• Protein number
  P09874
• Alternate protein number
  Please refer to SwissProt
• Gene number
  142
• Gene name
  Poly ADP Ribose Polymerase
• Alternate gene name
  PARP1; ADPRT; ADPRT1; PPOL; pADPRT-1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1
• Estimated production time
  7-11 business days
• Shipping requirements
  Blue ice
• Storage recommendation
  -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
• Use for
  8 months
• ELISA s specificity
  This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.
• ELISA s cross reactivity
  This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
• Assay principle
  The microplate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.;
• Protocol
  Please see ELISA's datasheet, otherwise contact us
• Precision of the test
  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
• ELISA s stability
  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
• QC
  The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
• Research main area
  Enzyme & Kinase;
• Warnings
  Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Description
  A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

• Gene target
  Poly   ADP   Ribose   Polymerase   PARP   CLIA   Kit  
• Gene symbol
  WDTC1, ADPGK, TNKS2, TIPARP, PARP1, PARP12, TNKS
• Short name
  Poly ADP Ribose Polymerase (PARP) CLIA Kit, 96
• Species
  Human, Humans
• Alternative name
  H. sapiens Poly ADP Ribose polymerizing enzyme (PARP) CLIA reagent, 96 well plate
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:29175
• Gene
  WDTC1
• Long gene name
  WD and tetratricopeptide repeats 1
• Synonyms
  • KIAA1037
  • ADP
  • DCAF9
• Synonyms name
  • adipose homolog (Drosophila)
  • DDB1 and CUL4 associated factor 9
• GenBank acession
  • AK023778
• Locus
  1p36.11
• Discovery year
  2003-12-22
• Entrez gene record
  23038
• Pubmed identfication
  • 12717455
  • 19238144
• RefSeq identity
  • NM_015023
• Classification
  • WD repeat domain containing
  • Tetratricopeptide repeat domain containing
  • DDB1 and CUL4 associated factors
• VEGA ID
  OTTHUMG00000004273

Gene info

• Identity
  HGNC:25250
• Gene
  ADPGK
• Long gene name
  ADP dependent glucokinase
• Synonyms
  • DKFZp434B195
  • ADP-GK
• GenBank acession
  • AL136873
• Locus
  15q24.1
• Discovery year
  2004-09-29
• Entrez gene record
  83440
• Pubmed identfication
  • 11230166
• RefSeq identity
  • NM_031284
• VEGA ID
  OTTHUMG00000172777

Gene info

• Identity
  HGNC:15677
• Gene
  TNKS2
• Long gene name
  tankyrase 2
• Synonyms gene name
  • tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
• Synonyms
  • TNKL
  • TANK2
  • PARP-5b
  • PARP-5c
  • PARP5B
  • PARP5C
  • pART6
  • ARTD6
• Synonyms name
  • TRF1-interacting ankyrin-related ADP-ribose polymerase 2
• GenBank acession
  • AF342982
• Locus
  10q23.32
• Discovery year
  2001-05-10
• Entrez gene record
  80351
• RefSeq identity
  • NM_025235
• Classification
  • Poly(ADP-ribose) polymerases
  • Sterile alpha motif domain containing
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000018747

Gene info

• Identity
  HGNC:23696
• Gene
  TIPARP
• Long gene name
  TCDD inducible poly(ADP-ribose) polymerase
• Synonyms gene name
  • TCDD-inducible poly(ADP-ribose) polymerase
• Synonyms
  • DKFZP434J214
  • DKFZp686N0351
  • DDF1
  • PARP7
  • PARP-7
  • PARP-1
  • pART14
  • RM1
  • ARTD14
• GenBank acession
  • BX537965
• Locus
  3q25.31
• Discovery year
  2003-12-02
• Entrez gene record
  25976
• Pubmed identfication
  • 12851707
• RefSeq identity
  • NM_015508
• Classification
  • Poly(ADP-ribose) polymerases
• VEGA ID
  OTTHUMG00000158646

Gene info

• Identity
  HGNC:270
• Gene
  PARP1
• Long gene name
  poly(ADP-ribose) polymerase 1
• Synonyms gene
  • PPOL
  • ADPRT
• Synonyms gene name
  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
  • poly (ADP-ribose) polymerase family, member 1
• Synonyms
  • PARP
  • ARTD1
• GenBank acession
  • BC037545
• Locus
  1q42.12
• Discovery year
  1986-01-01
• Entrez gene record
  142
• Pubmed identfication
  • 10964595
• RefSeq identity
  • NM_001618
• Classification
  • Zinc fingers PARP-type
  • Zinc fingers
  • Poly(ADP-ribose) polymerases
• VEGA ID
  OTTHUMG00000037556

Gene info

• Identity
  HGNC:21919
• Gene
  PARP12
• Long gene name
  poly(ADP-ribose) polymerase family member 12
• Synonyms gene
  • ZC3HDC1
• Synonyms gene name
  • zinc finger CCCH-type domain containing 1
• Synonyms
  • FLJ22693
  • PARP-12
  • ZC3H1
  • ARTD12
• GenBank acession
  • AL136766
• Locus
  7q34
• Discovery year
  2003-12-10
• Entrez gene record
  64761
• Pubmed identfication
  • 11230166
  • 12851707
• RefSeq identity
  • NM_022750
• Classification
  • Poly(ADP-ribose) polymerases
  • Zinc fingers CCCH-type
• VEGA ID
  OTTHUMG00000157315

Gene info

• Identity
  HGNC:11941
• Gene
  TNKS
• Long gene name
  tankyrase
• Synonyms gene name
  • tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• Synonyms
  • TIN1
  • TINF1
  • TNKS1
  • PARP-5a
  • PARP5A
  • pART5
  • ARTD5
• Synonyms name
  • TRF1-interacting ankyrin-related ADP-ribose polymerase 1
• GenBank acession
  • AF082556
• Locus
  8p23.1
• Discovery year
  1999-01-08
• Entrez gene record
  8658
• Pubmed identfication
  • 9822378
  • 10198177
• RefSeq identity
  • NM_003747
• Classification
  • Poly(ADP-ribose) polymerases
  • Sterile alpha motif domain containing
  • Ankyrin repeat domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000090481

Product images

Similar products