Hamster Anti-Mouse CD103-PE

• Catalog number
  MBS673588
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [CD103 (Integrin alpha IEL)]
• Products_name_syn
  [Hamster Anti-Mouse CD103 - Phycoerythrin-Labeled; Hamster Anti-Mouse CD103 (Integrin alpha IEL)]
• Clonality
  Monoclonal
• Clone
  [2E7]
• Reactivity
  Mouse
• Specificity
  The CD103/ alpha IEL beta 7 integrin complex (Mr 160 and 115 kDa peptides). CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery.1-3 In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells. The 2E7 mAb exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290.1 The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 This co-stimulatory activity is also shared by the mAb M290.4 The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.5
• Form
  R - phycoerythrin (R - PE) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD103-PE  
• Gene symbol
  ETV3, ITGAE, ERF
• Short name
  Anti-Mouse CD103-PE
• Technique
  anti-, Mouse, anti, antibody to, antibodies, mouses
• Host
  Armenian Hamster
• Isotype
  Armenian Hamster IgG
• Label
  PE
• Species
  Mouse, Hamsters, Mouses
• Alternative name
  Hamster Antibody toMouse CD103-peroxidase
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:3492
• Gene
  ETV3
• Long gene name
  ETS variant transcription factor 3
• Synonyms gene name
  • ets variant gene 3, ETS family transcriptional repressor
  • ETS variant 3
• Synonyms
  • PE-1
• GenBank acession
  • BC022868
• Locus
  1q23.1
• Discovery year
  1994-12-15
• Entrez gene record
  2117
• Pubmed identfication
  • 8020980
• RefSeq identity
  • NM_005240
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000034298

Gene info

• Identity
  HGNC:6147
• Gene
  ITGAE
• Long gene name
  integrin subunit alpha E
• Synonyms gene name
  • integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• Synonyms
  • CD103
  • HUMINAE
• Synonyms name
  • antigen CD103
  • human mucosal lymphocyte antigen 1, alpha polypeptide
• GenBank acession
  • L25851
• Locus
  17p13.2
• Discovery year
  1997-06-09
• Entrez gene record
  3682
• Pubmed identfication
  • 8119947
• RefSeq identity
  • NM_002208
• Classification
  • CD molecules
  • Integrin alpha subunits
• VEGA ID
  OTTHUMG00000177634

Gene info

• Identity
  HGNC:3444
• Gene
  ERF
• Long gene name
  ETS2 repressor factor
• Synonyms gene name
  • Ets2 repressor factor
• Synonyms
  • PE-2
  • PE2
• GenBank acession
  • U58535
• Locus
  19q13.2
• Discovery year
  1998-07-17
• Entrez gene record
  2077
• Pubmed identfication
  • 7588608
  • 9192842
• RefSeq identity
  • NM_006494
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000182788

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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