cAMP Direct Immunoassay Kit (Colorimetric)

• Catalog number
  K371-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay
• Summary
  • Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. BioVision’s cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  cAMP   Direct   Immunoassay   Kit  
• Short name
  cAMP Direct Immunoassay Kit (Colorimetric)
• Technique
  colorimetric, immunoassay, colorimetry
• Alternative name
  cathelicidin antimicrobial peptide Direct Immunoassay reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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