Human CellExp™ IL-2 R alpha / CD25, Mouse Recombinant
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Catalog numberP1464-10
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PricePlease ask
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Size10 µg
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SynonymsIL2RA, CD25, p55, IL2-RA, IL-2-RA
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Alternative_namesIL2RA, CD25, p55, IL2-RA, IL-2-RA
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DescriptionExtracellular Domain of IL-2R fused to 6x His tag at the C-terminus.
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RecombinantTrue
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SourceHEK 293 cells
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Purity by SDS PAGE>90%
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AssaySDS-PAGE
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Endotoxin LevelLess than 1.0 EU per ug by the LAL method.
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Biological activityImmobilized Biotinylated Human IL-2, Fc Tag, A at 5 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate, can bind Mouse IL-2 R alpha with a linear range of 5-40 ng/mL. Immobilized Mouse IL-2 R alpha at 5 ug/mL (100 uL/well) can bind Biotinylated Human IL-2 with a linear range of 0.6-10 ng/mL
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Molecular WeightThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized
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Reconstitution InstructionsReconstitute in sterile deionized water to the desired protein concentration.
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Background InformationInterleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
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Amino acid sequenceGlu 22 - Lys 236
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HandlingCentrifuge the vial prior to opening.
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Additional descriptionThe CellExp™ IL-2 R alpha / CD25, Mouse Recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolIL2, ISG20, IL2RA, IL36G, IL25, IL22, IL18, IL37, IL1RAPL2, IL6R
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Short nameCellExp™ IL-2 R alpha / CD25, Mouse Recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesHumans, Mouses
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Alternative nameH. sapiens CellExp™ Interleukin-2 R a / CD25, Mouse Rec.
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene nameinterleukin 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterferon stimulated exonuclease gene 20
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Synonyms gene name
- interferon stimulated exonuclease gene 20kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-01-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Exonucleases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 2 receptor subunit alpha
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Synonyms gene
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Synonyms gene name
- insulin-dependent diabetes mellitus 10
- interleukin 2 receptor, alpha
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Sushi domain containing
- Interleukin receptors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 36 gamma
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Synonyms gene
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Synonyms gene name
- interleukin 1 family, member 9
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 25
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Synonyms gene
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Synonyms gene name
- interleukin 17E
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-26
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Pubmed identfication
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 22
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 18
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Synonyms gene name
- interleukin 18 (interferon-gamma-inducing factor)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-07-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 37
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Synonyms gene
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Synonyms gene name
- interleukin 1 family, member 7 (zeta)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 1 receptor accessory protein like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- TIR domain containing
- Immunoglobulin like domain containing
- Interleukin receptors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 6 receptor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Interleukin receptors
- Immunoglobulin like domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data