Human CellExp™ IL-2 R alpha / CD25, Mouse Recombinant

  • Catalog number
    P1464-10
  • Price
    Please ask
  • Size
    10 µg
  • Synonyms
    IL2RA, CD25, p55, IL2-RA, IL-2-RA
  • Alternative_names
    IL2RA, CD25, p55, IL2-RA, IL-2-RA
  • Description
    Extracellular Domain of IL-2R fused to 6x His tag at the C-terminus.
  • Recombinant
    True
  • Source
    HEK 293 cells
  • Purity by SDS PAGE
    >90%
  • Assay
    SDS-PAGE
  • Endotoxin Level
    Less than 1.0 EU per ug by the LAL method.
  • Biological activity
    Immobilized Biotinylated Human IL-2, Fc Tag, A at 5 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate, can bind Mouse IL-2 R alpha with a linear range of 5-40 ng/mL. Immobilized Mouse IL-2 R alpha at 5 ug/mL (100 uL/well) can bind Biotinylated Human IL-2 with a linear range of 0.6-10 ng/mL
  • Molecular Weight
    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized
  • Reconstitution Instructions
    Reconstitute in sterile deionized water to the desired protein concentration.
  • Background Information
    Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
  • Amino acid sequence
    Glu 22 - Lys 236
  • Handling
    Centrifuge the vial prior to opening.
  • Additional description
    The CellExp™ IL-2 R alpha / CD25, Mouse Recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    CellExp™   IL-2   alpha   CD25  
  • Gene symbol
    IL2, ISG20, IL2RA, IL36G, IL25, IL22, IL18, IL37, IL1RAPL2, IL6R
  • Short name
    CellExp™ IL-2 R alpha / CD25, Mouse Recombinant
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    mouse
  • Species
    Humans, Mouses
  • Alternative name
    H. sapiens CellExp™ Interleukin-2 R a / CD25, Mouse Rec.
  • Alternative technique
    rec, murine
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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