Synaptotagmin Antibody
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Catalog number3177-100
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PricePlease ask
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Size100 µg
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Antibody TargetSynaptotagmin
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Target Alternative NameSynaptotagmin-1, Synaptotagmin I, SytI, p65, Syt1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 298 of rat Synaptotagmin
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AccessionP21707
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Gene Id25716
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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FormulationIn phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
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PurificationAffinity purified
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ApplicationWestern blot
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Positive ControlRat kidney tissue lysate
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Application And UsagesThe antibody can be used in Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3521BP-50) is available separately.
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DescriptionThe synaptotagmins are integral membrane proteins of synaptic vesicles thoμght to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; neurexins, syntaxin and AP2.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSYT16, SYT9, SYT3
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Short nameSynaptotagmin Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameSynaptotagmin (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namesynaptotagmin 16
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Synonyms gene
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Synonyms gene name
- synaptotagmin XIV-like
- synaptotagmin XVI
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-09-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Synaptotagmins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptotagmin 9
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Synonyms gene name
- synaptotagmin IX
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GenBank acession
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Locus
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Discovery year2004-10-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Synaptotagmins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptotagmin 3
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Synonyms gene name
- synaptotagmin III
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GenBank acession
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Locus
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Discovery year1995-09-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Synaptotagmins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data