For quantitative measurement of human BMP-5 in cell culture supernatant, serum and bone tissue.
ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.056 + 0.099 + 0.116 + 0.201 + 0.348 + 0.634 + 1.135 + 1.946
ELISA kit detection limits
156pg/ml to 10000g/ml
ELISA's sensitivity< 10pg/ml
Target of this ELISA
Natural and recombinant human BMP-5
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.23;3.46;6.92;1.38;3.57;6.85 ELISA standard deviations 0.059;0.180;0.388;0.073;0.203;0.425 CV(%);4.8;5.2;5.6;5.3;5.7;6.2
ELISA kit description
GENTAUR's human BMP-5 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for BMP-5 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A317-H454) and your samples are measured with a biotin conjugated polyclonal from goat specific for BMP-5 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human BMP-5 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human BMP-5 antibodies;1 ST0000-10;Lyophilized recombinant human BMP-5 standard;10ng/tube×2 AR1107;Biotinylated anti- human BMP-5 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human BMP-5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or bone tissue to the ELISA plate. Keep in mind that each human BMP-5 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
More gene info
Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Bone morphogenetic protein 5
bone morphogenetic protein 5
BMP-5;Bmp5;BMP5_HUMAN;Bone morphogenetic protein 5
Gen protein identity
Expression system for standard: NSO; Immunogen sequence: A317-H454
Storage & Transport Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
There is no detectable cross-reactivity with other relevant proteins.
cell culture supernates, serum and bone tissue.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
BMP-5 PicoKine ELISA Kit
ELISA kit, ELISA
antigen assay ELISA kit 1 Kit (96 Wells)
H. sapiens BMP-5 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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