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Human BMP 5 PicoKine ELISA Kit

Human BMP 5 PicoKine ELISA Kit is available 2 times from Boster labs

EK0310 | Human BMP-5 PicoKine ELISA Kit size: 1x96 well plate | 662.43 USD

Catalog number EK0310
Supplier boster
Price 662.43 USD
Size 1x96 well plate
1. Gene info
Identity1072
GeneBMP5
Locus6p12.1
Discovery year1991-06-05
Pubmed identfication
Havana BLAST/BLATOTTHUMG00000014903
MeSH Data
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Product images
    #Human#.jpg

EK0310 | Human BMP-5 PicoKine ELISA Kit size: 96wells/kit, with removable strips. | 610.28 USD

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ELISA's application For quantitative measurement of human BMP-5 in cell culture supernatant, serum and bone tissue.
Gene tested Human BMP-5
ELISA standard curve Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.056 + 0.099 + 0.116 + 0.201 + 0.348 + 0.634 + 1.135 + 1.946
ELISA kit detection limits 156pg/ml to 10000g/ml
ELISA's sensitivity< 10pg/ml
Target of this ELISA Natural and recombinant human BMP-5
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.23;3.46;6.92;1.38;3.57;6.85 ELISA standard deviations 0.059;0.180;0.388;0.073;0.203;0.425 CV(%);4.8;5.2;5.6;5.3;5.7;6.2
ELISA kit description GENTAUR's human BMP-5 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for BMP-5 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A317-H454) and your samples are measured with a biotin conjugated polyclonal from goat specific for BMP-5 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human BMP-5 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- human BMP-5 antibodies;1 ST0000-10;Lyophilized recombinant human BMP-5 standard;10ng/tube×2 AR1107;Biotinylated anti- human BMP-5 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation /
How the elisa works Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human BMP-5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or bone tissue to the ELISA plate. Keep in mind that each human BMP-5 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation 25-30
More gene info Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
Antigen NSO, A317-H454
Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot Bone morphogenetic protein 5
Gene name bone morphogenetic protein 5
Alternative BMP-5;Bmp5;BMP5_HUMAN;Bone morphogenetic protein 5
Gen protein identity P22003
Entrez nr. 653
Gene Name BMP5
Assay range 156pg/ml-10,000g/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: A317-H454
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
Kit Components 10ng/tube
Sample Type cell culture supernates, serum and bone tissue.
Applications ELISA
Reactivity Human
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetBMP-5 PicoKine
Short name BMP-5 PicoKine ELISA Kit
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative name H. sapiens BMP-5 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Human BMP-5 PicoKine ELISA Kit size: 1x96 well plate
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