Mouse Anti-Human VAMP1/2 Monoclonal IgG1, Clone SP-11
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Catalog numberSMC-179C
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PricePlease ask
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Size25 µg
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CloneSP-11
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ImmunogenCrude human synaptic immunoprecipitate
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Antibody s full descriptionMouse Anti-Human VAMP Monoclonal IgG1 Antibody, Clone: SP-11: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameSYB1 Antibody, Synaptobrevin Antibody, vesicle associated membrane protein Antibody
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Raised inMouse
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Antibody s targetVAMP
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ELISA
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Antibody s reactivityHuman, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~20-25kDa. Recognizes VAMP 1 and 2.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-179 was sufficient for detection of VAMP in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMitochondrion , Mitochondrion Outer Membrane , Cytoplasmic Vesicle
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextVAMP-1, also known as vesicle-associated membrane protein, is an 18kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3).
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Bibliography1. Trimble W.S., Cowan D.M., and Scheller R.H. (1988) Proc Natl Acad Sci USA. 85: 4538-4542. 2. Nabokina S.M. (2001) Bulletin of Experimental Bio and Med. 131(2): 130-131. 3. Berglund L., Hoffman H.J., Dahl R., and Peterson T.E. (1999) Biochem Biophys Research Communicaitons. 264(3): 777-780.
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Released date29-Jun-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_055046.1
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Gene number6843
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Protein numberP23763
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11 (SMC-179). Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody (SMC-179) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Fibers and Neurons. Immunohistochemistry analysis using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11 (SMC-179). Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody (SMC-179) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Brain neurons with lack of staining on the blood vessels. Magnification: 20x. Western Blot analysis of Rat brain lysates showing detection of VAMP protein using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11 (SMC-179). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody (SMC-179) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-VAMP Antibody [SP-11] used in Immunohistochemistry (IHC) on Human Cerebellum (SMC-179) Mouse Anti-VAMP Antibody [SP-11] used in Immunohistochemistry (IHC) on Human Brain Slice (SMC-179) Mouse Anti-VAMP Antibody [SP-11] used in Western Blot (WB) on Rat brain lysates (SMC-179)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.025
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Gene target
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Gene symbolSFTPA1, WDR87, PIRC52, IGKV1OR2-11, PIRC51, VAMP1, REXO5, SPATA1, SPESP1
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Short nameMouse Anti- VAMP1/2 Monoclonal IgG1, Clone SP-11
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Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelUnconjugated
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SpeciesHuman, Humans, Mouses
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Alternative nameMouse Antibody toHuman VAMP1/2 monoclonal IgG1, clonality SP-11
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Alternative techniquemurine, antibodies
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Clone nameSP-11
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Gene info
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Identity
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Gene
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Long gene namesurfactant protein A1
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Synonyms gene
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Synonyms gene name
- surfactant, pulmonary-associated protein A1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Collectins
- C-type lectin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWD repeat domain 87
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-07-06
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Entrez gene record
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- WD repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 52
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-11 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1/OR2-11
- immunoglobulin kappa variable 1/OR2-11 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 51
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namevesicle associated membrane protein 1
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Synonyms gene
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Synonyms gene name
- synaptobrevin 1
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Synonyms
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Locus
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Discovery year1990-03-14
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Entrez gene record
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Pubmed identfication
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Classification
- Vesicle associated membrane proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA exonuclease 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2017-03-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namespermatogenesis associated 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-03-01
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesperm equatorial segment protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
