MGMT (888CT22.2.4) Monoclonal Antibody

• Catalog number
  bsm-51234M
• Price
  Please ask
• Size
  0.1ml

• Long name
  MGMT (888CT22.2.4) Monoclonal Antibody
• Also known as
  MGMT (888CT22.2.4) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  MGMT
• Specificity
  This is a highly specific antibody against MGMT.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  888CT22.2.4
• Source
  Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
• Gene ID number
  4255
• Swiss Prot
  P16455
• Tested Applications
  WB, IHC-P, IF
• Recommended dilutions
  IHC-P(1:25), IF(1:25),WB(1:1000)
• Cross reactivity
  Human
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
• Purification method
  Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  MGMT   888CT22   Monoclonal  
• Gene symbol
  MGMT
• Short name
  Anti-MGMT
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1
• Alternative name
  MGMT (888CT22.2.4) Monoclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  O-6-methylguanine-DNA methyltransferase, MGMT and IDBG-93188 and ENSG00000170430 and 4255, DNA-methyltransferase activity, nuclei, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314

Gene info

• Identity
  HGNC:7059
• Gene
  MGMT
• Long gene name
  O-6-methylguanine-DNA methyltransferase
• Synonyms name
  • methylated-DNA--protein-cysteine methyltransferase
• GenBank acession
  • M29971
• Locus
  10q26.3
• Discovery year
  1989-10-16
• Entrez gene record
  4255
• RefSeq identity
  • NM_002412
• Classification
  • Methyltransferases
• VEGA ID
  OTTHUMG00000019261

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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