HiScipt II One Step RT-PCR Kit (Dye Plus), 50 tests (50 μl/tests)
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Availability
In Stock. Please contact us to check the latest availability or to request a quote for larger or bulk quantity
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Product utilization
The product HiScipt II One Step RT-PCR Kit (Dye Plus)is designed for application in One Step RT-PCR analyses
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Storage conditions
Our specialists from Gentaur/Genprice recommend you to follow stricktly the instructions for storage of the product HiScipt II One Step RT-PCR Kit (Dye Plus) described in the manual to keep the quality and stability of the reagents.
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Tips
This product is manufactured for research purposes only. Please, contact our agents to check if there is any information about its application in diagnostics.
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Group
PCR, polymerase chain reaction, RT-PCR mixes
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
HiScipt II One Step RT-PCR Kit (Dye Plus), 50 (50 μl/ )
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Technique
RT-PCR, step, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. steps
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Label
dye
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Alternative name
HiScipt II One Step Reverse transcription PCR test kit reagent (Dye Plus), 50 tests (50 μl/tests)
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Alternative technique
rtpcrkits, steps, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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