DNA Bisulfite Conversion Kit I
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Catalog number
K1478-200
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Price
Please ask
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Size
200 Rxns
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Description
Spin Column based bisulfite DNA purification
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Summary
Bisulfite modification of DNA and purification using spin columns
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Detection Method
Bisulfite modification of DNA and purification using spin columns
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Applications
An ideal tool for bisulfite modification of DNA and purification
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Sample Type
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. High-quality and RNA-free DNA is required.
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Features Benefits
Many downstream applications such as Next generation Sequencing, PCR amplification, etc.,High recovery of DNA,Results ready in less than 2 h,Reliable and Ready-to-use,High quality spin columns to recover bisulfite-treated DNA
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Storage Conditions
-20°C
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Shipping Conditions
RT
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Shelf life
12 months
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Background
BioVision’s DNA Bisulfite Conversion Kit I is used for the bisulfite conversion of DNA that can be used to study the expression 5-methylcytosine. 5-methylcytosine is an epigenetic marker that plays an important role in differentiation, neurodegeneration, cancer etc. In this kit, DNA is treated with sodium bisulfite, which leads to the deamination of cytosine residues and conversion to uracil, while 5-methyl cytosine residues remain unaffected. DNA Bisulfite Conversion Kit I integrates the DNA denaturation and bisulfite conversion processes into a single step. The kit has been developed for the high recovery of DNA following DNA bisulfite conversion. This kit provides innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers with consistent results every time. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosine residues. The DNA recovered from this kit is used for downstream applications such as endonuclease digestion, Next Generation Sequencing, microarrays, PCR amplification, etc.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
DNA Bisulfite Conversion Kit I
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Technique
dna
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Alternative name
Desoxyribonucleic acid Bisulfite Conversion reagent I
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
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