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Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
For cells, cell lines and tissues in culture till half confluency.Isotype or positive controls by peptides, antibodies and deactivated samples.cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
Western Blot, Recombinant, Control, Blot
Alternative to gene target
keratin 18, CYK18 and K18, KRT18 and IDBG-35299 and ENSG00000111057 and 3875, scaffold protein binding, nuclei, Krt18 and IDBG-187416 and ENSMUSG00000023043 and 16668, KRT18 and IDBG-631626 and ENSBTAG00000001517 and 506480