Human Recombinant PPP1CA

• Catalog number
  6370-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Human Recombinant PPP1CA
• Alternative_names
  Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A.
• Description
  Crucial for cell division
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥80%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: >3,000 units/mg.
• Results
  >3,000 units/mg
• Unit Definition
  One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
• Molecular Weight
  39.7 kDa (350 aa, 1-330 aa + NT His-Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.25 mg/ml
• Appearance
  Liquid
• Physical form description
  0.25 mg/ml solution in 50 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 1 mM DTT, 0.15 M NaCl, 1 mM MnCl2 and 0.1 mM PMSF.
• Background Information
  Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PPP1CA  
• Gene symbol
  PPP1CA
• Short name
  Recombinant PPP1CA
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. PPP1CA
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9281
• Gene
  PPP1CA
• Long gene name
  protein phosphatase 1 catalytic subunit alpha
• Synonyms gene
  • PPP1A
• Synonyms gene name
  • protein phosphatase 1, catalytic subunit, alpha isoform
  • protein phosphatase 1, catalytic subunit, alpha isozyme
• Synonyms
  • PP1A
  • PP-1A
  • PP1alpha
• Locus
  11q13.2
• Discovery year
  1989-10-11
• Entrez gene record
  5499
• RefSeq identity
  • NM_002708
• Classification
  • Protein phosphatase catalytic subunits
• VEGA ID
  OTTHUMG00000167671

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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