Donkey anti Goat IgG H + L Alexa Fluor 594
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Catalog number
43R-ID005AF
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Price
Please ask
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Size
500 µg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Donkey anti-goat IgG (H + L) (Alexa Fluor 594) was raised in donkey using goat IgG (H & L) ..
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Raised in
Donkey
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Specificity
Goat whole IgG
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Cross Reactivity
Minimal crossreaction with Human, Mouse, Chicken, Rabbit, Guniea Pig, Syrian Hamster, Horse, Rat
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Conjugate Tag
Alexa Fluor 594
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Method of Purification
Donkey anti Goat IgG (H + L) (Alexa Fluor 594) was purified by affinity chromatography.
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Concentration
1.5 mg/ml
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Form Buffer
Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3. Reconstitute with 0.4 ml of distilled Water.
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Storage
Store lyophilized at 4 deg C. Reconstitute in 400 ul dH2O, and store for up to 6 weeks at -20 deg C or aliquot and store at -70 deg C long term.
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Shipping conditions
Blue Ice
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Tested for
WB; IFA; IF; ELISA
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Usage Recommendations
Use at 1:100-1:800 for most applications
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin name
Equus africanus asinus, Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
anti Goat IgG H + L
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Technique
anti, Goat, IgG, antibody to, goats, IgGs
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Host
Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
ALEXA FLUOR 594
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Species
Donkeys, Goats
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Alternative name
Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)
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Alternative technique
antibodies, caprine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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