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Synonyms
CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15
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Alternative_names
CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15
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Description
Transmembrane glycoprotein that controls innate and adoptive immune response.
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Recombinant
True
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Source
HEK 293 cells
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Purity by SDS PAGE
>90%
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Assay
SDS-PAGE
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Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
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Biological activity
Immobilized Mouse Siglec-15 at 5 ug/mL (100 uL/well) can bind Mouse Human chimeric MAb with a linear range of 0.3-2 ng/mL
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Molecular Weight
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
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Amino acid sequence
Arg 24 - Thr 262
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Handling
Centrifuge the vial prior to opening.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
