Active Cathepsin B, human recombinant

  • Catalog number
    7580-50
  • Price
    Please ask
  • Size
    50 ug
  • Synonyms
    CTSB, CPSB
  • Alternative_names
    CTSB, CPSB
  • Description
    A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    >7500 mU/mg (1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100)).
  • Biological activity
    >7500 mU/mg (1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100)).
  • Results
    >7500 mU/mg
  • Unit Definition
    1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100).
  • Molecular Weight
    27.8 kDa (80-333 aa)
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Concentration
    0.5 mg/ml
  • Appearance
    Liquid
  • Physical form description
    0.5 mg/ml solution of protein in 25 mM sodium acetate, 50 mM NaCl, pH 5.0 containing 50% glycerol.
  • Background Information
    Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene
    CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    Active Cathepsin B, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    functionnal Cathepsin B, H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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