DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES

Storage

+2°C to +8°C
Product Group

Classical Media
Shelf Life

18 months
Buffer

25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

Gene target

DMEM Glucose 5 g/l with Stable Glutamine with
Short name

DMEM Glucose (4 5 g/l), with Stable Glutamine, with 25 mM
Alternative name

DMEM High Glucose (4.5 g/l), including Stable Glutamine, including 25 millimeter HEPES