H3K27me3S28p polyclonal antibody

  • Catalog number
    6810-50
  • Price
    Please ask
  • Size
    50 μl
  • Antibody Target
    H3K27me3S28p
  • Target Alternative Name
    Histone H3
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human.
  • Immunogen Sequence
    KLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28.
  • Accession
    P68431
  • Gene Id
    8350
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In PBS with 0.05% (W/V) sodium azide.
  • Purification
    Whole antiserum from rabbit
  • Application
    Western blot, IF, ELISA, ChIP, Dot blot.
  • Positive Control
    Western blot: HeLa cells, IF: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
  • Application And Usages
    IF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP.
  • Description
    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Verified
  • ChIP Verified
    Verified
  • Dot Blot Verified
    Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
  • Short name
    H3K27me3S28p polyclonal antibody
  • Technique
    Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Isotype
    Rabbit IgG
  • Alternative name
    H3K27me3S28p polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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