H3K27me3S28p polyclonal antibody
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Catalog number6810-50
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PricePlease ask
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Size50 μl
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Antibody TargetH3K27me3S28p
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Target Alternative NameHistone H3
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Antibody TypePolyclonal
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Species ReactivitiesHuman.
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Immunogen SequenceKLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28.
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AccessionP68431
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Gene Id8350
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.05% (W/V) sodium azide.
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PurificationWhole antiserum from rabbit
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ApplicationWestern blot, IF, ELISA, ChIP, Dot blot.
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Positive ControlWestern blot: HeLa cells, IF: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
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Application And UsagesIF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP.
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DescriptionHistones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedVerified
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Dot Blot VerifiedVerified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Short nameH3K27me3S28p polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeRabbit IgG
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Alternative nameH3K27me3S28p polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data