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Immunogen
Synthetic peptide from the mid-protein of Human Ghrelin
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Antibody s target
Human Ghrelin
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Antibody s full description
Rabbit Anti-Human Ghrelin Polyclonal
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Primary research fields
Cardiovascular System, Blood, Cardiovascular System, Atherosclerosis, Diabetes Associated, Neuroscience, Cardiovascular System, Angiogenesis, Growth Factors
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Appetite-regulating hormone Antibody, ghrl Antibody, Ghrelin 28 Antibody, Protein M46 Antibody, GHRL_HUMAN Antibody, GHRL Antibody, Ghrelin Antibody, Growth hormone-releasing peptide Antibody, Motilin-related peptide Antibody, Appetite regulating hormone Antibody, Growth hormone releasing peptide Antibody, Growth hormone secretagogue Antibody, Motilin related peptide Antibody, Ghrelin/obestatin prepropeptide Antibody, Obestatin Antibody, MTLRP Antibody, Ghrelin 27 Antibody, M46 protein Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~13 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-696 was sufficient for detection of Ghrelin in 15 µg of rat Pancreas cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Secreted
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Tissue specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_001128413.1
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Gene number
51738
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Protein number
Q9UBU3
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~12.9 kDa. Rabbit Anti-Ghrelin Antibody used in Western blot (WB) on Pancreas (SPC-696)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
