FITC antibody
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Catalog numberGEN531027
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PricePlease ask
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Size1000ug
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Also known asFITC
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Other namesFitC; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameFITC
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG2a
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CloneM25286
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Host organismMouse (Mus musculus)
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Species reactivityN/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityN/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearancePBS, pH 7.4, with 0.1% NaN3.
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ConcentrationN/A
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Storage and shippingFor shorter periods of time keep the antibody at +4 degrees Celsius. Long term storage should be done after aliquoting and at -20°C. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies, thus reducing their activity.
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Tested applicationsN/A
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Short nameFITC antibody
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameAnti-FITC antibody
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Alternative techniqueantibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data