Target of this antibody:
Alternative names of antibody target:
ROCK1 , P160ROCK , MGC131603 , MGC43611 , p160ROCK , EC 220.127.116.11
ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to the caspase-3 cleavage site
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) Protein G purified mouse anti-human ROCK1 monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate treated with camptothecin (2 µM for 6-8 hours) can be used as a positive control. The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
anti-ROCK1 Antibody (Clone 12M03)
antibody to-Rho-associated, coiled-coil containing protein kinase 1 (Antibody to) (clonality 12M03)
Alternative to gene target
Rho-associated, coiled-coil containing protein kinase 1, P160ROCK and ROCK-I, ROCK1 and IDBG-1371 and ENSG00000067900 and 6093, transferase activity, Plasma membranes, Rock1 and IDBG-128359 and ENSMUSG00000024290 and 19877, ROCK1 and IDBG-644080 and ENSBTAG00000008403 and 785911