-
ELISA s application
For quantitative measurement of mouse GDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
-
Gene tested
murine GDNF
-
ELISA standard curve
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.010 + 0.069 + 0.099 + 0.195 + 0.389 + 0.790 + 1.469 + 2.305
-
ELISA kit detection limits
31.2pg/ml to 2000pg/ml
-
ELISA s sensitivity
< 10pg/ml
-
Target of this ELISA
Natural and recombinant mouse GDNF
-
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
-
ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);138;716;1354;146;727;1379 ELISA standard deviations 6.07;36.52;86.66;10.51;53.8;113.1 CV(%);4.4;5.1;6.4;7.2;7.4;8.2
-
ELISA kit description
GENTAUR's mouse GDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for GDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, S78-I211) and your samples are measured with a biotin conjugated polyclonal from goat specific for GDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse GDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
-
Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
-
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse GDNF antibodies;1 ST0000-10;Lyophilized recombinant mouse GDNF standard;10ng/tube×2 AR1107;Biotinylated anti- mouse GDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
-
Anti coagulation
heparin or EDTA
-
How the elisa works
Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse GDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse GDNF standard solution and each sample be measured in duplicate.
-
Minutes of TMB incubation
25-30
-
More gene info
Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
-
Antigen
sf21, S78-I211
-
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
-
Uniprot
Glial cell line-derived neurotrophic factor
-
Gene name
glial cell derived neurotrophic factor
-
Alternative
Astrocyte derived trophic factor;ATF;ATF1;ATF2;Glial cell line derived neurotrophic factor;HFB1 GDNF;hGDNF;HSCR3
-
Gen protein identity
P48540
-
Entrez nr
14573
-
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
-
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
Gene target
-
Gene symbol
GDNF
-
Short name
Mouse GDNF PicoKine ELISA Kit
-
Technique
ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
-
Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
-
Species
Mouse, Mouses
-
Alternative name
Mouse glial cell derived neurotrophic factor PicoKine Enzyme-linked immunosorbent assay reagent
-
Alternative technique
murine, elisas, kits
-
Alternative to gene target
glial cell derived neurotrophic factor, ATF1 and ATF2 and HFB1-GDNF and HSCR3, GDNF and IDBG-17169 and ENSG00000168621 and 2668, protein homodimerization activity, Extracellular, Gdnf and IDBG-128378 and ENSMUSG00000022144 and 14573, BT.58072 and IDBG-630047 and ENSBTAG00000005176 and 386587
-