Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ELISA's application For quantitative measurement of mouse GDNF in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
More gene info Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
Anti-coagulation heparin or EDTA
Kit Components 10ng/tube
Uniprot Glial cell line-derived neurotrophic factor
Gen protein identity P48540
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
Immunogen Expression system for standard: sf21; Immunogen sequence: S78-I211
Gene tested murine GDNF
ELISA standard curve Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.010 + 0.069 + 0.099 + 0.195 + 0.389 + 0.790 + 1.469 + 2.305
Gene name glial cell derived neurotrophic factor
ELISA kit description GENTAUR's mouse GDNF ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for GDNF was used as capure antibodies on the ELISA test plate. The internal standards (sf21, S78-I211) and your samples are measured with a biotin conjugated polyclonal from goat specific for GDNF will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse GDNF in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation 25-30
How the elisa works Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse GDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse GDNF standard solution and each sample be measured in duplicate.
Alternative Astrocyte derived trophic factor;ATF;ATF1;ATF2;Glial cell line derived neurotrophic factor;HFB1 GDNF;hGDNF;HSCR3
Entrez nr. 14573
ELISA kit detection limits 31.2pg/ml to 2000pg/ml
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse GDNF antibodies;1 ST0000-10;Lyophilized recombinant mouse GDNF standard;10ng/tube×2 AR1107;Biotinylated anti- mouse GDNF antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Antigen sf21, S78-I211
Gene Name Gdnf
Assay range 31.2pg/ml-2000pg/ml
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Target of this ELISA Natural and recombinant mouse GDNF
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);138;716;1354;146;727;1379 ELISA standard deviations 6.07;36.52;86.66;10.51;53.8;113.1 CV(%);4.4;5.1;6.4;7.2;7.4;8.2
Detector and capure antibodies A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus
Short name Mouse GDNF PicoKine ELISA Kit
Technique ELISA kit, ELISA, Mouse
Alternative name Mouse glial cell derived neurotrophic factor PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target glial cell derived neurotrophic factor, ATF1 and ATF2 and HFB1-GDNF and HSCR3, GDNF and IDBG-17169 and ENSG00000168621 and 2668, protein homodimerization activity, Extracellular, Gdnf and IDBG-128378 and ENSMUSG00000022144 and 14573, BT.58072 and IDBG-630047 and ENSBTAG00000005176 and 386587, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832