Protein A Agarose IP-Ready antibody Antibody Purification Reagent

• Catalog number
  X1203
• Price
  Please ask
• Size
  1,5mL

• Category
  Other Reagents
• Long description
  ExalphaÕs IP-Readyª agarose suspension products contain 0.5 ml of agarose conjugate and are ready to use for immunoprecipitation procedures. They are provided preblocked with fatty acid free bovine serum albumin and have been extensively washed. Formulations are ready to use, 10 µl of agarose per 1 µg of primary antibody used in standard immunoprecipitation procedures.
• Antibody come from
  n/a
• Other description
  Supplied 0.5 ml of preblocked Protein A agarose in 1 ml of phosphate buffered saline containing 0.08% sodium azide.
• Clone
  not specified
• Antigen antibody binding interaction
  Protein A Agarose IP-Ready antibody Antibody Purification Reagent
• Antibody is raised in
  see techfile
• Antibody s reacts with
  IgG
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Immunoprecipitation
• Antibody s suited for
  Basic procedure for immunoprecipitation assay using IP-Readyª products. These are basic guidelines and may need to be modified for your particular application:_x000B_Prep of PBSTDS: Mix 100 ml of 10x PBS with 10 ml of 100% Triton X-100; 5 g of sodium deoxycholate and 1.0 g sodium dodecyl sulfate. Include a cocktail of protease inhibitors, such as 1 mM EDTA; 1 µg/ml Pepstatin and 0.2 mM PMSF from 100 mM stock in 2-propanol, or a cocktail of you choosing; Dilute this solution to 1 L with distilled water._x000B__x000B_1. Preclear lysate or media by adding ~1 µg of normal IgG from the same species from which the primary antibody is derived to remove nonspecific proteins._x000B_2. Incubate for 1 hour at 4¼C and then centrifuge at 1,500 rpm reserving supernatant for specific IP._x000B_3. Determine total protein of the sample._x000B_4. To a 1.5 ml Eppendorf tube, add the following: Cell lysate (1 mg total protein), 1 µg of purified primary antibody and 10 µl of packed beads._x000B_5. Cap tubes and incubate at 4¼C on rocker platform or rotating device for 2 to 24 hours._x000B_6. Centrifuge at 2,500 rpm for 15 minutes at 4oC and collect immunoprecipitate._x000B_7. Wash pellet 4 times with 1.0 ml of PBSTDS, repeating centrifuge step above (step 6) each time._x000B_8. After final wash, aspirate and discard supernatant and resuspend pellet in 40-50 µl of_x000B_electrophoresis sample buffer._x000B_9. Analyze samples by either SDS-PAGE or transfer to a membrane and detect by Western blot._x000B__x000B_
• Storage
  4ºC
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Properties
  The purest agarose was used in the production of Protein A Agarose IP- antibody Antibody Purification Reagent by nordc. If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Agarose
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
• French translation
  anticorps

• Gene target
  Protein   Reagent  
• Short name
  Protein A Agarose IP- antibody Antibody Purification Reagent
• Technique
  Purification, Antibody, Agarose, IP, agaroses, antibodies against human proteins, antibodies for, Immunoprecipitation, purified
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Protein A molecular sieve IP-Ready (antibody to-) (antibody to-) purification Reagent
• Alternative technique
  purifications, antibodies, separation

MeSH Data

• Name
  Chromatography, Agarose
• Concept
  Scope note: A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
• Tree numbers
  • E05.196.181.400.250.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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