Protein A Agarose antibody Antibody Purification Reagent

  • Catalog number
    X1191
  • Price
    Please ask
  • Size
    1mL
  • Category
    Other Reagents
  • Long description
    This outstanding recombinant Protein A is coupled through a cysteine at the carboxy terminus creating an ether linkage to highly cross-linked agarose. It contains 6 mg of recombinant Protein A per ml of beads. The combinantion provides superior performance, binding 50 mg of human IgG per ml of beads under static conditions. Binding under flow will be less depending on the flow rate for all Protein A and Protein G products. Flow rate of 30 to 300 cm/hr are possible with this highly cross-linked agarose product under gravity flow, but it is not designed to pump under high pressure.
  • Antibody come from
    n/a
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide.
  • Clone
    not specified
  • Antigen antibody binding interaction
    Protein A Agarose antibody Antibody Purification Reagent
  • Antibody is raised in
    see techfile
  • Antibody s reacts with
    IgG
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    IgG Purification
  • Antibody s suited for
    Ligand Density: approximately 6 mg Protein A per ml of drained gel _x000B_Dynamic Binding Capacity: approximately 35 mg human IgG per ml of drained gel (estimated in a 0.7x5cm column at a linear flow rate of 300 cm/h under the following conditions: binding buffer: 0.1 M PBS pH 7.0, Eluting buffer 0.1 M citrate pH 3.0. 2.8 times excess IgG of the binding capacity was applied. _x000B_Bead Structure: 4% highly cross-linked agarose _x000B_Bead Size Range: 45 to 165 µm _x000B_Mean Bead Size: approximately 90 µm _x000B_Maximum Linear Flow Rate: >1300 cm/hour at 25 deg C in an XK 16/20 column, bed height 5 cm _x000B_pH Stability: Long term storage - 3 to 9; short term storage - 2 to 10 _x000B_Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 40 ¼ C in 3M NaSCN, 6M guanidine-HCl, 0.1M glycine (pH3.0), 70% ethanol; 10 hours at 23¼ C in 8M urea, 0.1M glycine (pH 11), 5.8M acetic acid (pH 2.0) _x000B_Physical stability: Negligible volume variation due to changes in pH or ionic strength _x000B_Sanitization: Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
  • Storage
    4ºC
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Properties
    The purest agarose was used in the production of Protein A Agarose antibody Antibody Purification Reagent by nordc. If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation
    Agarose
  • Test
    Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
  • French translation
    anticorps
  • Gene target
  • Short name
    Protein A Agarose antibody Antibody Purification Reagent
  • Technique
    Purification, Antibody, Agarose, agaroses, antibodies against human proteins, antibodies for, purified
  • Isotype
    not specified
  • Label
    unlabeled
  • Alternative name
    Protein A molecular sieve (antibody to-) (antibody to-) purification Reagent
  • Alternative technique
    purifications, antibodies, separation
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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