NFkB-p65 (K303) polyclonal antibody
-
Catalog number
BS3157-50ul
-
Price
Please ask
-
Size
50ul
-
-
Alternative Name
p65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3
-
Immunogen
Synthetic peptide, corresponding to amino acids 280-320 of Human NFkB-p65.
-
Swiss Prot
Q04206
-
Reactivity
Human,Mouse,Rat
-
Applications
WB IHC IF
-
Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
-
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
-
Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
Specificity
NFκB-p65 (K303) polyclonal antibody detects endogenous levels of NFκB-p65 protein.
-
Usage
Western blot (WB) analysis of NFkB-p65 (K303) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(32ug) Lane2:RAW264.7 whole cell lysate(32ug) Lane3:H9C2 whole cell lysate(19ug) Lane4:Hela whole cell lysate(32ug)
-
BiowMW
~ 60, 75 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Short name
NFkB-p65 (K303) polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit
-
Alternative name
NFkB-p65 (K303) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products