MLH1 Antibody

• Catalog number
  F41625-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MLH1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, FACS, ELISA
• Recommended dilutions
  Western blot: 1:1000,Flow Cytometry: 1:10-1:50
• Notes
  Titration of the MLH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MLH1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P40692
• Purity
  Antigen affinity
• Description
  This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
• Immunogen
  A portion of amino acids 452-480 from the human protein was used as the immunogen for this MLH1 antibody.
• Storage
  Aliquot the MLH1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MLH1  
• Gene symbol
  MLH1
• Short name
  Anti-MLH1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to MLH1
• Alternative technique
  antibodies
• Alternative to gene target
  mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, structure-specific DNA binding, nuclei, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652

Gene info

• Identity
  HGNC:7127
• Gene
  MLH1
• Long gene name
  mutL homolog 1
• Synonyms gene
  • COCA2
• Synonyms gene name
  • mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Synonyms
  • HNPCC
  • FCC2
  • HNPCC2
• GenBank acession
  • U07418
• Locus
  3p22.2
• Discovery year
  1993-11-24
• Entrez gene record
  4292
• Pubmed identfication
  • 7903889
• RefSeq identity
  • NM_000249
• Classification
  • MutL homologs
• VEGA ID
  OTTHUMG00000130797
• Locus Specific Databases
  • Hereditary Non-Polyposis Colorectal Cancer, HNPCC
  • Global Variome shared LOVD
  • MLH1 database at LOVD-China
  • LOVD - Leiden Open Variation Database
  • LRG_216

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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