Alternative names of antibody target:
BDNF; Brain-derived neurotrophic factor; Abrineurin
Background information:
Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
Clone:
N/A
Immunogene sequence:
This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.
Concentration of antibody:
N/A
Formulation of this antibody:
100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Purification method:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
This antibody has been succesfully tested for use in following methods:
Western blot, IHC
Recommended usage:
Western blot: ~1:1000, IHC: ~1:10–1:50, IF: ~1:10–1:50, FC: ~1:10–1:50.
Identity 1033
Gene BDNF
Long gene name brain derived neurotrophic factor
Locus 11p14.1
Discovery year 1991-01-15
Entrez gene record 627
Pubmed identfication- 2236018
- 1889806
- 17942328
- 17493809
Havana BLAST/BLAT OTTHUMG00000178797
Identity 8754
Gene PCYT1A
Long gene name phosphate cytidylyltransferase 1, choline, alpha
Synonyms gene name- phosphate cytidylyltransferase 1, choline, alpha isoform
Synonyms name- phosphate cytidylyltransferase 1, choline, alpha isoform
Locus 3q29
Discovery year 1999-05-05
Entrez gene record 5130
Havana BLAST/BLAT OTTHUMG00000155670
