BDNF Antibody (CT)
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Catalog number6725-100
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PricePlease ask
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Size100 μl
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Antibody TargetBDNF
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Target Alternative NameBDNF; Brain-derived neurotrophic factor; Abrineurin
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceThis BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.
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AccessionP23560
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Gene Id627
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl of antibody in PBS with 0.09% (W/V) sodium azide
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PurificationThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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ApplicationWestern blot, IHC
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Positive ControlWestern blot: CEM cell line, IHC: human brain tissue, FACS: CEM cells, IF: NCI-H460 cells
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Application And UsagesWestern blot: ~1:1000, IHC: ~1:10–1:50, IF: ~1:10–1:50, FC: ~1:10–1:50.
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DescriptionNeurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedVerified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBDNF, BDNF-AS
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Short nameBDNF Antibody (CT)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namebrain-derived neurotrophic factor (antibody to-) (CT)
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Alternative techniqueantibodies
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Alternative to gene targetbrain-derived neurotrophic factor, ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, growth factor activity, Extracellular, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701
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Gene info
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Identity
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Gene
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Long gene namebrain derived neurotrophic factor
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-01-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neurotrophins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBDNF antisense RNA
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Synonyms gene
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Synonyms gene name
- BDNF opposite strand (non-protein coding)
- BDNF antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-02-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data