Mouse IgG2a ELISpotBASIC kit (ALP)

Categories

ELISpot Kits
Description

With this kit, detection of antigen-specific IgG2a secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody, which is available in the web shop.
Enzyme Conjugate

NA
Format

without plates
Kit Detection Type

Streptavidin-ALP
Application

ELISpot
Reactivities

mouse
Analytes

IgG2a
Contents

Capture mAbs (MT24/JC5-1),Biotinylated detection mAb (MG2a),Streptavidin-ALP,R848,Recombinant mouse IL-2
Datasheet
Product Header

Basic ELISpot for enumeration of IgG2a secreting B cells
GeneID

NA
Shelf Life

18 months
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

IgG2a ELISpotBASIC kit ALP
Gene symbol

PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
Short name

Mouse IgG2a ELISpotBASIC kit (ALP)
Technique

Mouse, mouses
Host

mouse
Isotype

IgG2a
Species

Mouse, Mouses
Alternative name

Mouse IgG2a ELISpotBASIC reagent (ALP)
Alternative technique

murine, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:11092
Gene

SLPI
Long gene name

secretory leukocyte peptidase inhibitor
Synonyms gene name
- secretory leukocyte protease inhibitor (antileukoproteinase)
Synonyms
- HUSI-I
- ALK1
- ALP
- BLPI
- HUSI
- WAP4
- WFDC4
Synonyms name
- antileukoproteinase
GenBank acession
- X04502
Locus

20q13.12
Discovery year

1998-04-24
Entrez gene record

6590
Pubmed identfication
- 3640338
- 12206714
Classification
- WAP four-disulfide core domain containing
VEGA ID

OTTHUMG00000033075

Identity

HGNC:10626
Gene

CCL27
Long gene name

C-C motif chemokine ligand 27
Synonyms gene
- SCYA27
Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 27
- chemokine (C-C motif) ligand 27
Synonyms
- ALP
- ILC
- CTACK
- skinkine
- ESkine
- PESKY
- CTAK
Synonyms name
GenBank acession
- AJ243542
Locus

9p13.3
Discovery year

1999-07-30
Entrez gene record

10850
Pubmed identfication
- 10556532
- 10588729
RefSeq identity
- NM_006664
Classification
- Chemokine ligands
VEGA ID

OTTHUMG00000019834

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data