JNK Activated Cell Lysate

Description

Anisomycin activated Jurkat cells extract for a positive control in western blot and JNK activity assays. Jurkat cells are cultured in RPMI medium supplement with 10% FBS. The cells were grown to late growing phase. Anisomycin is added to the medium to a final concentration 10 µg/ml (BioVision cat# 1549-10, dissolved in DMSO to 10 mg/ml as stock). The cells are incubated at 37°C for 15 minutes, then collected and lysed in Extraction Buffer (BioVision Cat # K430-40). The cell lysate can be used as a positive control for JNK activity assays in conjunction with JNK Activity Assay Kit (K430-40 or K431-40). For positive controls, we recommend using 100 µg per assay.
Product Highlights

• CONCENTRATION- 4 mg/ml
Storage Temp

-70°C
Shipping

dry ice
Shelf Life

6 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.
Additional description

For cells, cell lines and tissues in culture till half confluency.